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KMID : 0545119960060010036
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Journal of Microbiology and Biotechnology
1996 Volume.6 No. 1 p.36 ~ p.42
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Enzymatic Sorbitol Production with Zymomonas mobilis Immobilized in k-Carrageenan
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JANG, KI-HYO
JUNG, SUNG-JE/CHANG, HYUN-SOO/CHUN, UCK-HAN
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Abstract
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The production of sorbitol by permeabilized cells of Zymomonas mobilis immobilized in κ-carrageenan was investigated. Cetyltrimethylammoniumbromide (CTAB) permeabilized cells were treated with glutaraldehyde prior to immobilization for cross-linking of enzymes, glucose-fructose oxidoreductase (GFOR) in cells. Rigidity of the immobilized beads was increased two-fold with 90% conversion efficiency by the additions of 40% (w/v) polyols (glycerol 25 g + propylene glycol 15 g) to 60% (w/v) distilled water containing 2.5% (w/v) κ-carrageenan as a final concentration, prior to immobilization. κ-Carrageenan beads entrapping permeabilized cells were dried to improve bead rigidity and storage stability. During semi-batch process for 72 h with dry beads, there was an improvement of the loss of enzyme activity (less than 10%). In batch process, the kinetic results of K_m.fructose value for the free cells, wet obtained with two-stage continuous packed bed reactors with both wet and dry κ-carrageenan beads at 25.00 and 21.15 g/l/h, respectively, when measured at second stage.
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